primary antibodies against α sma  (Bimake Inc)

Journal: Bioengineering

Article Title: Photothermolysis with 1550 nm Fractional Laser Promotes Regeneration of Gingival Mucosa

doi: 10.3390/bioengineering12111180

Figure Lengend Snippet: α-smooth muscle actin in intact gingical tissue ( a , b ) areas treated with 70 kJ ( c , d ), 100 kJ ( e , f ) and 130 kJ ( g , h ) lasers six weeks after treatment. Immunohistochemical reaction with antibodies against α-smooth muscle actin with hematoxylin counterstaining, magnification ×100 ( left column ), ×200 ( right column ), arrow—healing laser treatment area, star—neoangiogenesis.

Article Snippet: Four-μm-thick sections of the formalin-fixed-paraffin-embedded tissue samples were deparaffinized, incubated with 3% H 2 O 2 for 10 min, underwent heat induced epitope retrieval (pH 6.0 sodium citrate buffer, 30 min in 80 °C water bath), additionally blocked with Background Block (Cell Marque, Rocklin, CA, USA) and incubated with mouse monoclonal primary antibodies against α-smooth muscle actin (α-SMA) (A2547, Merck, Rahway, NJ, USA, diluted 1:400) and detected by horseradish peroxidase-conjugated secondary goat antibodies (G-21040, Invitrogen, Carlsbad, CA, USA, diluted 1:1000) and diaminobenzidine with hematoxylin counterstaining.

Techniques: Immunohistochemical staining